24 research outputs found
Antioxidants of natural plant origins: from sources to food industry applications
ReviewIn recent years, great interest has been focused on using natural antioxidants in food
products, due to studies indicating possible adverse effects that may be related to the consumption of
synthetic antioxidants. A variety of plant materials are known to be natural sources of antioxidants,
such as herbs, spices, seeds, fruits and vegetables. The interest in these natural components is
not only due to their biological value, but also to their economic impact, as most of them may be
extracted from food by-products and under-exploited plant species. This article provides an overview
of current knowledge on natural antioxidants: their sources, extraction methods and stabilization
processes. In addition, recent studies on their applications in the food industry are also addressed;
namely, as preservatives in different food products and in active films for packaging purposes and
edible coatingsinfo:eu-repo/semantics/publishedVersio
Application of an Eco-Friendly Antifungal Active Package to Extend the Shelf Life of Fresh Red Raspberry (Rubus idaeus L. cv. ‘Kweli’)
The main objective of this study was to extend the shelf life of fresh red raspberry
(Rubus idaeus. L. cv. ‘Kweli’) by using active film-pads inside commercial compostable packages. The
pads were produced with chitosan (Ch) with the incorporation of green tea (GTE) and rosemary
(RSME) ethanolic extracts as natural antifungal agents. Pads were placed on the bottom of commercial
fruit trays underneath the fruits, and the trays were heat-sealed with a polyacid lactic (PLA) film.
Preservation studies were carried out over 14 days of storage at refrigeration temperature (4 C).
Raspberry samples were periodically analyzed throughout storage, in terms of quality attributes
(fungal decay, weight loss, firmness, surface color, pH, total soluble solids), total phenolic content
and antioxidant activity. Gas composition inside the packages was also analyzed over time. From
the packaging systems tested, the ones with active film-pads Ch + GTE and Ch + RSME were highly
effective in reducing fungal growth and decay of raspberry during storage, showing only around
13% and 5% of spoiled fruits after 14 days, respectively, in contrast with the packages without pads
(around 80% of spoiled fruits detected). In addition, fruits preserved using packages with Ch + RSME
active film-pads showed lower mass loss (5.6%), decreased firmness (3.7%) and reduced antioxidant
activity (around 9% and 15% for DPPH and FRAP methods, respectively). This sustainable packaging
presents a potential strategy for the preservation of raspberries and other highly perishable
small fruitsinfo:eu-repo/semantics/publishedVersio
Design of chitosan and alginate emulsion-based formulations for the production of monolayer crosslinked edible films and coatings
This study aimed to develop edible monolayer emulsion-based barriers with polysaccharides
as film-forming components (chitosan and sodium alginate), soy lecithin as a surfactant and
olive oil as a hydrophobic barrier. Monolayer barriers in the form of films were prepared by casting
filmogenic emulsions composed of 2% w/v chitosan (dissolved in lactic acid 1% v/v) or 1% w/v
sodium alginate, with different lipid contents (25, 50 and 100% w/w biopolymer basis) and different
surfactant concentrations (5, 10 and 25% w/w, lipid basis). Glycerol was used as a plasticizer (25 %
w/w, biopolymer basis). After the emulsion drying process, the obtained stand-alone films were
sprayed with a crosslinking solution, achieving an optimized crosslinker content of 3.2 mgCa2+/cm2
alginate film and 4 mg tripolyphosphate/cm2 chitosan film. The effect of oil and lecithin contents,
as well the presence of crosslinking agents, on the film’s water vapour permeability (WVP), water
vapour sorption capacity, mechanical properties and colour parameters, was evaluated. The results
have shown that the lowest WVP values were obtained with formulations containing 25% lipid and
25% surfactant for chitosan films, and 100% lipid and 25% surfactant for alginate films. The application
of the crosslinking agents decreased even further the WVP, especially for chitosan films (by 30%).
Crosslinking also increased films’ resistance to deformation under tensile tests. Overall, the films
developed present a good potential as polysaccharide-based barriers with increased resistance to
water, which envisages the use of the designed formulations to produce either edible/biodegradable
films or edible coatingsinfo:eu-repo/semantics/publishedVersio
Composite coatings of chitosan and alginate emulsions with olive oil to enhance postharvest quality and shelf life of fresh figs (Ficus carica L. cv. Pingo De Mel)
Fresh figs are very appreciated and have been associated with health benefits. However,
these fruits are highly perishable. In this study, edible coatings were studied envisaging their positive
effect in enhancing figs’ shelf-life. Fig fruits cv. ‘Pingo de mel’ were harvested at commercial ripening
stage and single emulsion-based coatings, composed of chitosan + olive oil and alginate + olive oil,
were applied. After coatings application by dipping each fruit in the emulsion-based solutions at 4 C
and drying, the coated fruits were sprayed with crosslinking solutions (6% tripolyphosphate and 1%
calcium chloride for chitosan and alginate-based coatings, respectively). Then, were maintained at
4 C and analyzed after 1, 7, 14 and 19 days of storage. After each time interval, fruits were further
maintained at 25 C for 2 days. The results have shown that coatings were effective on delaying
fungal decay and postharvest ripening indicators (respiration rate, mass loss, softening and total
soluble solids/titratable acidity ratio). The results foresee a fruits’ shelf life between 14 and 19 days
under refrigeration at 4 C that may be followed up to 2 days at ambient temperature, higher than
that estimated for uncoated fruits (less than 14 days at 4 C plus to 2 days at ambient temperature)info:eu-repo/semantics/publishedVersio
Influence of a heat-shock pre-treatment on wound-induced phenolic biosynthesis as an alternative strategy towards fresh-cut carrot processing
In fresh-cut vegetables, plant tissues are often challenged by (a)biotic stresses that act in combination, and the
response to combinatorial stresses differs from that triggered by each individually. Phenolic induction by
wounding is a known response contributing to increase products phenolic content. Heat application is a
promising treatment in minimal processing, and its interference on the wound-induced response is
produce-dependent. In carrot, two-combined stress effects were evaluated: peel removal vs. shredding,
and heat application (100 C/45 s) vs. shredding, on changes in total phenolic content (TPC) during 10
days (5 C). By applying the first stress combination, a decrease in TPC was verified on day 0 ( 50%),
ascribed to the high phenolic content of peels. Recovery of initial fresh carrot levels was achieved after 7
days owing to phenolic biosynthesis induced by shredding. For the second combination, changes in TPC,
phenylalanine-ammonia-lyase (PAL), and peroxidase (POD) activity of untreated (Ctr) and heat-treated (HS)
peeled shredded carrot samples were evaluated during 10 days. The heat-shock did not suppress phenolic
biosynthesis promoted by PAL, although there was a two-day delay in TPC increments. Notwithstanding,
phenolic accumulation after 10 days exceeded raw material TPC content. Also, the decrease in POD activity
(30%) could influence quality degradation during storageinfo:eu-repo/semantics/publishedVersio
Impact of chitosan-beeswax edible coatings on the quality of fresh strawberries (Fragaria ananassa cv Camarosa) under commercial storage conditions
Chitosan-based edible coatings were used to prolong the shelf-life of strawberries stored at 20 C and 35
e40% RH. Strawberries were coated with four different coating formulations (chitosan as monolayer,
three layer coating consisting of separate beeswaxechitosanebeeswax layers, three layer coating where
chitosan was crosslinked with sodium tripolyphosphate, TPP, and composite). The effectiveness of the
coatings was evaluated by the changes of several parameters: fungal infection, weight loss, respiration
rate, skin and flesh color, firmness, pH value, titratabale acidity, soluble solids content, reducing sugars
content and sensory evaluation. The coatings, especially the three-layer coatings, significantly decreased
the senescence and weight loss of the fruits. They modified the respiration rates of the strawberries and
slowed down their metabolism as shown by the retention of the color and the texture of the tissue.
Sensory evaluation of the coated strawberries showed that the chitosan and composite coatings gave
better visual appearance and taste and were therefore more preferable by 90% of the judges than the
three-layer coatings, even though the later had higher protective effect of the overall quality of the
strawberrie
The effect of calcium dips combined with mild heating of whole kiwifruit for fruit slices quality maintenance
The effect of moderate heat treatment combined with calcium dips on the quality of minimally processed kiwifruit was studied. Whole
fruits were treated for 25 min at 45 C by dipping in deionised water or CaCl2 solutions (1%, 2% and 3% (w/v)) and cooled to 4 C.
Twenty-four hours later fruits were peeled, sanitized, cut into slices and packed. The firmness of kiwifruit slices’ was subsequently evaluated
during 8 days of storage. Calcium content, pectinmethylesterase activity and heat shock proteins accumulation were also investigated.
Heat treatment conducted in water induced a firming effect and avoid softening of fruit slices while calcium dips had a marginal
effect on this parameter. A calcium loss was observed due to dip treatment, but this effect was minimized when treatment was conducted
in 3% CaCl2 solution. The firming effect provided is due to the activation of pectinmethylesterase and the presence of calcium in treatment
solution reduces or inhibits enzyme activation. Under the tested conditions, no heat shock proteins de novo synthesis was detecte
Impacto do microencapsulamento na estabilidade do corante natural obtido a partir dos subprodutos do morango e do mirtilo
V Colóquio Nacional da Produção de Pequenos Frutos. Sessão III - Qualidade e pós-colheitaOs pequenos frutos como o mirtilo e o morango muitas vezes não podem ser comercializáveis para fresco devido à sua elevada perecibilidade, podendo constituir matéria-prima para extração de corantes naturais para fins alimentares. Estes frutos têm na sua constituição antocianinas que são pigmentos responsáveis pela cor vermelha ou azul e que apresentam elevada atividade antioxidante. Contudo, a sua utilização como corantes tem sido limitada devido à baixa estabilidade durante o processamento e armazenamento dos alimentos em que são aplicados. Neste trabalho estudou-se o efeito da temperatura e da luz nas caracterÃsticas dos extratos aquosos de morango e mirtilo. Para estudar o efeito da microencapsulação na estabilidade da cor, procedeu-se à secagem dos extratos por liofilização e spray-drying utilizando a inulina como agente encapsulante. A análise dos resultados permite observar que a temperatura é a variável com efeito mais significativo na degradação das antocianinas e da cor dos extratos de morango e mirtilo, sendo que temperaturas mais elevadas conduzem a maior degradação. A microencapsulação por spray-drying e por liofilização altera significativamente a cor original dos extratos permitindo, no entanto, manter a respetiva cor ao longo do tempo de exposição à luz, contrariamente ao que se verifica com os extratos. A microencapsulação afigura-se promissora para a utilização de corantes naturais em alimentos processados pelo calor e/ou expostos à luzinfo:eu-repo/semantics/publishedVersio
Microencapsulation of beta-carotene by spray drying effect of wall material concentration and drying inlet temperature
Research ArticleCarotenoids are a class of natural pigments found mainly in fruits and vegetables. Among them, -carotene is regarded the most
potent precursor of vitamin A. However, it is susceptible to oxidation upon exposure to oxygen, light, and heat, which can result in
loss of colour, antioxidant activity, and vitamin activity.Thus, the objective of thiswork was to study themicroencapsulation process
of -carotene by spray drying, using arabic gum as wall material, to protect it against adverse environmental conditions. This was
carried out using the response surfacemethodology coupled to a central composite rotatable design, evaluating simultaneously the
effect of drying air inlet temperature (110-200∘C) and the wall material concentration (5-35%) on the drying yield, encapsulation
efficiency, loading capacity, and antioxidant activity. In addition, morphology and particles size distribution were evaluated.
Scanning electron microscopy images have shown that the particles were microcapsules with a smooth surface when produced
at the higher drying temperatures tested,most of them having a diameter lower than 10 m.The conditions that enabled obtaining
simultaneously arabic gummicroparticles with higher -carotene content, higher encapsulation efficiency, and higher drying yield
were a wall material concentration of 11.9% and a drying inlet temperature of 173∘C.The systematic approach used for the study of
-carotene microencapsulation process by spray drying using arabic gum may be easily applied for other core and wall materialsinfo:eu-repo/semantics/publishedVersio
Texture, microstructure and consumer preference of mango bars jellified with gellan gum
Studies for the development of a novel convenient fruit product based on mango puree and gellan gum
were carried out. Two gellan types (low acyl-L and high acyl-H) used in specific L/H ratios (75/25, 50/50,
25/75) for an overall concentration of 1 g of gellan/100 g of puree were tested, in order to design different
mango bars. The influence of the L/H gellan ratio on their texture properties (TPA and SR tests),
microstructure (Cryo-Scanning Electron Microscopy) and texture sensory acceptance (Preference-
Ranking test) was studied. The results obtained enabled to separate the bars in two groups: the ones
showing greater hardness and brittleness (only L gellan, and L/H at the ratios of 75/25 and 50/50); and
those presenting a softer structure with higher cohesiveness and springiness values (L/H of 25/75 and
only H gellan). The microstructure of mango bars was consistent with the texture results. Those presenting
higher hardness have shown a microstructure composed of a denser biopolymer network with
lower pore size; while for softer bars with higher springiness, larger pores and thin strings were
observed. From the Preference-Ranking test, the most appreciated mango bar in terms of texture was the
one prepared with a L/H ratio of 25/75.info:eu-repo/semantics/publishedVersio